Probes for improved melt discrimination and multiplexing in nucleic acid assays
US9982291B2 · kind B2 · utility
5Cited by
8References
13Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 11, 2015 |
| Grant date | May 29, 2018 |
| Priority date | — |
| Expiry date | Nov 20, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6823
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and/or quenching moiety.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.