System and method for monitoring particles in a fluid using ratiometric cytometry
US9983115B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 21, 2015 |
| Grant date | May 29, 2018 |
| Priority date | — |
| Expiry date | May 10, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06T2207/30188
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A particle detection system with a detection mechanism that includes detectors positioned to detect two different ranges of fluorescence produced by particles in the fluid in a flow chamber. Each of the detectors is arranged to generating a trigger signal whenever fluorescence is detected. The system and related method enhance the accuracy and sensitivity of blue-green algae monitoring by utilizing imaging flow cytometry combined with particle analysis and the measurement of the ratio of each particle's phycocyanin to chlorophyll b detected by using the two detectors configured for detection of two different fluorescence ranges, one associated with the phycocyanin and the other associated with the chlorophyll b. Captured images are be used in comparison to known images of a library of images using a support vector machine classifier.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.