Low-cost portable microfluidic system for cell migration
US9983197B2 · kind B2 · utility
Inventors
Key dates
| Filing date | Sep 10, 2013 |
| Grant date | May 29, 2018 |
| Priority date | — |
| Expiry date | Nov 27, 2034 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06T2207/30004
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Low-cost and portable microfluidic systems are needed for cell migration research and Point of Care (POC) testing. This study introduces a low-cost and portable USB Microscope Microfluidic Chemotaxis Analysis System (UMCAS) for rapid analysis of cell chemotaxis studies. A standalone microfluidic gradient generator is also developed for rapid generation of chemical gradient in microfluidic device without need of any peripheral perfusion apparatus. A smart phone based application program was developed for the real-time remote monitoring of the migration data. This system is validated by observing the neutrophil migration in three different conditions: 1) medium control, 2) uniform IL-8 control, and 3) IL-8 gradient. The results show that neutrophils exhibit random migration in both medium and uniform IL-8 control experiments, while they show strong directional migration to the IL-8 gradient. These results successfully validated the developed UMCAS system.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.