Rapid protein labeling and analysis
US9983211B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 25, 2016 |
| Grant date | May 29, 2018 |
| Priority date | — |
| Expiry date | Apr 25, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2550/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein. Following the labeling step, the proteins within the mixture can be separated and analyzed. In a further embodiment, a tag binding fluorogenic reagent that can bind to a tag on a tagged protein is added to specifically label the protein of interest.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.