Methods of seed breeding using high throughput nondestructive seed sampling

US9986699B2 · kind B2 · utility

Assignee

• Monsanto Technology LLC · US

Inventors

• Kevin L. Deppermann · Chesterfield, US
• Michael W. Petersen · Sauk City, US
• David Butruille · Urbandale, US
• Stanton Dotson · Chesterfield, US
• Sam Eathington · Ames, US
• Heather M. Forbes · Ankeny, US
• Bruce Schnicker · Wildwood, US
• John Tamulonis · Nevada, US

Key dates

• Filing date: Feb 23, 2015
• Grant date: Jun 5, 2018
• Expiry date: Aug 22, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/156
• WIPO field: Food chemistry
• WIPO sector: Chemistry

Abstract

Novel methods are provided to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. A method for introgressive hybridization, for example, generally includes removing tissue samples from individual seeds using an automated seed sampler without affecting germination viability of the seeds, and analyzing nucleic acids extracted from the tissue samples for at least one genetic marker. The method then further includes selecting the sampled seeds that possess the at least one genetic marker, cultivating fertile plants from the selected seeds, and crossing the fertile plants with other plants.