Large scale generation of functional megakaryocytes and platelets from human embryonic stem cells under stromal-free conditions
US9988603B2 · kind B2 · utility
10Cited by
3References
17Claims
0Family size
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Key dates
| Filing date | Dec 3, 2010 |
| Grant date | Jun 5, 2018 |
| Priority date | — |
| Expiry date | Jul 10, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2506/45
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention provides a method of generating megakaryocytes and platelets. In various embodiments, method involves the use of human embryonic stem cell derived hemangioblasts for differentiation into megakaryocytes and platelets under serum and stromal-free condition. In this system, hESCs are directed towards megakaryocytes through embryoid body formation and hemangioblast differentiation. Further provided is a method of treating a subject in need of platelet transfusion.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.