In vivo conversion of nucleosides in plasmid DNA
US9988659B1 · kind B1 · utility
0Cited by
7References
4Claims
0Family size
Inventors
Key dates
| Filing date | Mar 18, 2014 |
| Grant date | Jun 5, 2018 |
| Priority date | — |
| Expiry date | Feb 27, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2525/117
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The instant invention provides for the assembly of large DNA oligonucleotide constructs by the self-assembly of multiple oligonucleotide fragments, wherein the assembly is guided by the hybridization between non-standard nucleotides that form non-standard nucleobase pairs orthogonal to the standard T:A and C:G nucleobase pairs. Adding nucleobase pairs increases the information density of the fragments, minimizing off-target hybridization. The invention further provides rules and methods for converting non-standard pairs into standard pairs using polymerase copying with conversion.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.