Methods for determining protein binding specificity using peptide libraries
US9988665B2 · kind B2 · utility
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11Claims
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Key dates
| Filing date | Aug 12, 2011 |
| Grant date | Jun 5, 2018 |
| Priority date | — |
| Expiry date | Feb 19, 2033 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/98
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for determining protein binding specificity using a screen of a peptide library is provided. The method can be used to determine binding specificity for human NAD+-dependent deacetylase SIRT1, and to identify the most efficiently deacetylated peptide sequences. The method can be also used to screen a combinatorial H4 histone N-terminal tail peptide library to examine the binding preferences of a α-phos (S1) H4 antibody toward all known possible H4 histone modification states.
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