Modulation of structured polypeptide specificity
US9994617B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 8, 2012 |
| Grant date | Jun 12, 2018 |
| Priority date | — |
| Expiry date | Oct 8, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K7/64
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention describes a method for selecting a polypeptide ligand having a desired level of specificity for a target, wherein the polypeptide ligand comprises a polypeptide comprising at least three reactive groups, separated by at least two loop sequences, and a molecular scaffold which forms covalent bonds with the reactive groups of the polypeptide such that at least two polypeptide loops are formed on the molecular scaffold, comprising (i) screening at least two different sets of polypeptide ligands against the target, and selecting one or more ligands from each library which interact with the target; (ii) comparing the activity of the selected ligands with one or more paralogues or orthologues of the target; and (iii) further selecting one or more ligands according to their activity towards said one or more paralogues or orthologues; wherein said two or more different sets of ligands differ in the length of the polypeptide loops formed on the molecular scaffold.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.