Catalyst-free surface functionalization and polymer grafting

US9994687B2 · kind B2 · utility

Assignee

• Illumina, Inc. · US

Inventors

• Lorenzo Berti · San Diego, US
• Andrew A. Brown · Cambridge, GB
• Wayne N. George · London, GB

Key dates

• Filing date: Jun 26, 2014
• Grant date: Jun 12, 2018
• Expiry date: Jul 21, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C08J2335/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Some embodiments described herein relate to a substrate with a surface comprising a silane or a silane derivative covalently attached to optionally substituted cycloalkene or optionally substituted heterocycloalkene for direct conjugation with a functionalized molecule of interest, such as a polymer, a hydrogel, an amino acid, a nucleoside, a nucleotide, a peptide, a polynucleotide, or a protein. In some embodiments, the silane or silane derivative contains optionally substituted norbornene or norbornene derivatives. Method for preparing a functionalized surface and the use in DNA sequencing and other diagnostic applications are also disclosed.