Constructs for gene expression analysis
US9994858B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Oct 4, 2006 |
| Grant date | Jun 12, 2018 |
| Priority date | — |
| Expiry date | Jan 30, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/68
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates generally to constructs and their use in gene expression or gene regulation assays. More particularly, the present invention provides expression vectors and/or reporter vectors providing kinetics of protein expression with improved temporal correlation to promoter activity. Even more particularly, the invention provides expression vectors comprising a transcribable polynucleotide which comprises a sequence of nucleotides encoding a RNA element that modulates the stability of a transcript corresponding to the transcribable polynucleotide. The present invention provides, inter alia, novel vectors, useful for identifying and analysing cis- and trans-acting regulatory sequences/factors as well as vectors and genetically modified cell lines or organisms that are particularly useful for drug screening and drug discovery.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.