Floury 2 gene-specific assay in maize for floury (fl2) trait introgression

US9994919B2 · kind B2 · utility

Assignee

• DOW AGROSCIENCES LLC · US

Inventors

• Wei-Hao Chen · Yuanchang, TW
• Nathan Joel Van Opdorp · Geneseo, US
• Steve J. Plehn · Juneau, US
• Peter Friedemann · Philo, US
• Nadia Chaidir · Carmel, US
• Siva P. Kumpatla · Carmel, US

Key dates

• Filing date: May 29, 2013
• Grant date: Jun 12, 2018
• Expiry date: May 29, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/158
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The disclosure concerns compositions and methods for determining the zygosity of corn plants containing one or more floury2 (fl2) mutations. In embodiments, the disclosure concerns a gene specific PCR-based molecular (KASPar®) assay for identifying the floury2 trait in plants. In certain embodiments, compositions and methods are disclosed for determining the zygosity of corn plants with respect to the fl2 allele. In particular embodiments, the assay may be used for fl2 germplasm identification, accelerating introgression and molecular breeding programs in corn and other plants.