Method for the detection of enzymatic activity with magnetically functionalized substrates
US9999691B2 · kind B2 · utility
Inventor
Key dates
| Filing date | Dec 25, 2013 |
| Grant date | Jun 19, 2018 |
| Priority date | — |
| Expiry date | Nov 4, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/96425
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention provides methods for detecting an enzymatic activity, the method including combining at least one magnetic particle to an enzyme substrate to form a magnetically modified substrate, reacting the magnetically modified substrate with at least one enzyme; and detecting a change in a magnetic property of the magnetically modified substrate or its cleavage products, thereby detecting an activity of said at least one enzyme, wherein the method may be applied to a human subject to detect a disease selected from the group consisting of rheumatitis, arthritis, an injury, Dupuytren's disease, Peyronie's disease, a collagen related disease, steatosis, fibrosis, cirrhosis, metastasis, tissue regeneration, cancer, coronary disease, a liver disease, a metabolic condition, an infection and an inflammatory disease.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.