Patent · US Expired

Polypeptide cartilage-inducing factors found in bone

USRE34090E · kind E · reissue

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Assignee

Inventors

Key dates

Filing dateMar 5, 1991
Grant dateOct 6, 1992
Priority date
Expiry dateMar 5, 2011

Classification

  • Technology area (CPC —)General

Abstract

Two proteins that are found is bone and that have in vivo chondrogenic/osteogenic activity in combination with a co-factor are described. Both proteins also were active in combination with EGF in the in vitro TGF-.beta. assay. Each has a molecular weight of approximately 26,000 daltons by SDS-PAGE. Each is reduced to a single polypeptide indicating that the proteins are probably homodimers. One has an N-terminal sequence identical to that of human placenta-derived TGF-.beta. whereas the other has an N-terminal sequence that is different from that of TGF-.beta. derived from human placenta. The two proteins may be purified to homogeneity using RP-HPLC or acetic acid-urea gel electrophoresis.

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