Method for assaying proteolytic enzymes
USRE34284E · kind E · reissue
3Cited by
3References
5Claims
0Family size
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Key dates
| Filing date | Mar 9, 1992 |
| Grant date | Jun 15, 1993 |
| Priority date | — |
| Expiry date | Mar 9, 2012 |
Classification
- Technology area (CPC —)General
Abstract
A reagent for proteolytic enzyme assays has the general formula ##STR1## where RCO-- is an enzyme reactive acyl, such as an amino acid, peptide or substituted amino acid or peptide. The reagent may be hydrolysed by proteolytic enzymes and developed to form a distinctive color. The reagent may be formed by reacting RCOOH with N-hydroxysuccinimide to form the acyl N-hydroxysuccinimide ester. The ester may then be reacted to form the reagent.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.