Optimum fixed angle centrifuge rotor
USRE35071E · kind E · reissue
Assignee
Inventors
Key dates
| Filing date | Jun 18, 1993 |
| Grant date | Oct 24, 1995 |
| Priority date | — |
| Expiry date | Jun 18, 2013 |
Classification
- Technology area (CPC —)General
Abstract
A centrifuge rotor and method for gradient density separation which supports a generally cylindrical volume of sample solution with its axis inclined to the spin axis at an angle which is optimized for maximum separation efficiency while reducing contamination by undesirable centrifugates upon reorientation of the desirable centrifugates. .Iadd.The sample solution is contained in a centrifuge tube which is closed at its top end by a top portion which is supported by a support cap. The undesirable centrifugates are pelleted to the end corners of the inclined centrifuge tube. The rotor and method are particularly adapted, for example, for separation of nucleic acid into plasmid DNA and chromosomal DNA by density gradient centrifugation. .Iaddend.The optimum angle is determined based on the relationship .theta.=Tan.sup.-1 (D/15L).sup.0.5 where D and L are respectively the diameter and length of the cylindrical volume of the sample solution and .theta. is the angle of inclination.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.