Method for producing the Hinc II restriction endonuclease and methylase
USRE35248E · kind E · reissue
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13Claims
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Key dates
| Filing date | Jan 12, 1993 |
| Grant date | May 21, 1996 |
| Priority date | — |
| Expiry date | Jan 12, 2013 |
Classification
- Technology area (CPC —)General
Abstract
The present invention is directed to a method for cloning and producing the Hinc II restriction endonuclease by (1) introducing the restriction endonuclease gene from Haemophilus influenzae Rc into a host whereby the restriction gene is expressed; (2) fermenting the host which contains the vector encoding and expressing the Hinc II restriction endonuclease, and (3) purifying the Hinc II restriction endonuclease from the fermented host which contains the vector encoding and expressing the Hinc II restriction endonuclease activity.
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