Molecular genetic construction of vaccine strains of pasteurellaceae
USRE38028E1 · kind E1 · reissue
Assignees
Inventors
Key dates
| Filing date | Nov 21, 2000 |
| Grant date | Mar 11, 2003 |
| Priority date | — |
| Expiry date | Nov 21, 2020 |
Classification
- Technology area (CPC —)General
Abstract
Tools for genetically engineering Pasteurellaceae are provided. Replication-conditional plasmids which are useful for the Pasteurellaceae have been isolated and characterized. The plasmids can be utilized for delivery of DNA segments into the Pasteurellaceae in situations where control of extra-chromosomal replication desired, such as in achieving allelic exchange or site-directed mutagenesis.A restriction endonuclease, HsoI, was isolated from a bovine lung isolate of Haemophilus somnus. The enzyme was found to be a true isoschizomer of HinPI, a commercially available enzyme originally isolated from Haemophilus influenzae PI. Commercially available HhaI methyl transferase was found to protect against cleavage by both enzymes. Methylation of foreign plasmid DNA was found to enhance transformation of Haemophilus somnus in excess of four orders of magnitude.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.