Reagent for measurement of leukocytes and hemoglobin concentration in blood
USRE38131E1 · kind E1 · reissue
14Cited by
10References
6Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 15, 2001 |
| Grant date | Jun 3, 2003 |
| Priority date | — |
| Expiry date | Oct 15, 2021 |
Classification
- Technology area (CPC —)General
Abstract
A reagent for measurement of leukocytes and hemoglobin concentration in the blood includes a cationic surfactant in an amount sufficient to lyse erythrocytes and denature hemoglobin, at least one of the following hemoglobin stabilizers:(a) sulfosalicylic acid, or its salt, in an amount effective for promoting the conversion of hemoglobin into methemoglobin,(b) 0.2 to 10.0 g/L of a water-soluble chelating agent having a nitrogen atom and a carboxyl group, and(c) piperazine, or its salt, in an amount effective for promoting the conversion of hemoglobin into methemoglobin, anda buffer for maintaining pH at 4 to 6.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.