Hyperstabilizing antisense nucleic acid binding agents

USRE38169E1 · kind E1 · reissue

Assignee

• BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY AND AGRICULTURAL AND MECHANICAL COLLEGE · US

Inventor

• David Swenson · Midland, US

Key dates

• Filing date: Jul 26, 2000
• Grant date: Jul 1, 2003
• Expiry date: Jul 26, 2020

Classification

• Technology area (CPC —): General

Abstract

Attaching certain ligands to antisense probes will hyperstabilize sense-antisense duplexes. Such a hyperstabilized duplex is resistant to melting of the strands from one another, to unwinding of the strands, and to the action of nucleases. Applications include antiretroviral action, antireverse-transcriptase action, antiviral action, antiparasitical action, antibacterial action, antifungal action, anticancer action, anti-oncogene action, and other applications where it is desired to inhibit gene expression at the genomic or messenger RNA level. The preferred ligands are certain minor-groove-binding agents, exemplified by CC-1065 and synthetic CC-1065 analogs.