Haloalkyl derivatives of reporter molecules used to analyze metabolic activity in cells

USRE38723E1 · kind E1 · reissue

Assignee

• Molecular Probes, Inc. · US

Inventors

• Fei Mao · Fremont, US
• Ram Sabnis · Sacramento, US
• John J. Naleway · Eugene, US
• Nels Olson · Homer, US
• Richard P. Haugland · Eugene, US

Key dates

• Filing date: Aug 12, 1997
• Grant date: Apr 12, 2005
• Expiry date: Aug 12, 2017

Classification

• Technology area (CPC —): General

Abstract

The subject invention provides substrates useful for analyzing the metabolic activity in cells by improving the retention of a detectable reporter molecule only in intact cells where a particular enzyme is present. In particular, improved retention results from a two part process involving conjugation of haloalkyl-substituted derivatives of a reporter molecule with intracellular cysteine-containing peptides while unblocking the reporter molecule. The substrates have the formXR-SPACER-REPORTER-BLOCK