RNA amplification method requiring only one manipulation step
USRE39031E1 · kind E1 · reissue
Assignee
Inventors
Key dates
| Filing date | Oct 6, 2000 |
| Grant date | Mar 21, 2006 |
| Priority date | — |
| Expiry date | Oct 6, 2020 |
Classification
- Technology area (CPC —)General
Abstract
An RNA amplification method is particularly useful for diagnosing bacterial or viral infections or genetic disorders and for cell typing. The method includes the steps of denaturing a solution containing RNA, synthesizing a first cDNA strand from a suitable primer in the presence of reverse transcriptase, denaturing the heteroduplex formed, synthesizing a second cDNA strand from a second primer in the presence of DNA polymerase and then subjecting the cDNA formed to a sufficient number of amplification cycles. All the reactants and solvents are first placed in the same container to provide a single manipulation step that avoids the risk of contamination.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.