Luminescence by reacting an acridinium ester with superoxide

USRE39047E1 · kind E1 · reissue

Assignee

• MITSUBISHI KAGAKU IATRON, INC. · JP

Inventors

• Masuo Aizawa
• Eiry Kobatake · Tokyo, JP
• Minoru Ogasawara · Akita, JP
• Mitsunao Tanaka · Tokyo, JP
• Yoshihito Ikariyama · Tokyo, JP

Key dates

• Filing date: Mar 8, 2001
• Grant date: Mar 28, 2006
• Expiry date: Mar 8, 2021

Classification

• Technology area (CPC —): General

Abstract

A light emitting method of an acridinium ester, comprising reacting said acridinium ester and a superoxide anion, and a method of detecting a substance to be examined, comprising detecting a light emitted by reacting a superoxide anion with an acridinium ester used as a label am described. It is possible to carry out the reaction not under strongly alkaline conditions but around the neutral point and to generate strong luminescence which is stable over a long period of time.