Signal generation and mixing electronics for frequency-domain lifetime and spectral fluorometry
USRE39537E1 · kind E1 · reissue
Assignee
Inventors
Key dates
| Filing date | Nov 9, 2001 |
| Grant date | Apr 3, 2007 |
| Priority date | — |
| Expiry date | Nov 9, 2021 |
Classification
- Technology area (CPC —)General
Abstract
The present invention additionally comprises a method and apparatus for generating and mixing signals for frequency-domain lifetime and spectral fluorometry. The present invention comprises a plurality of signal generators that generate a plurality of signals where the signal generators modulate the amplitude and/or the frequency of the signals. The present invention uses one of these signals to drive an excitation signal that the present invention then directs and transmits at a target mixture, which absorbs the energy from the excitation signal. The property of fluorescence causes the target mixture to emit an emitted signal that the present invention detects with a signal detector. The present invention uses a plurality of mixers to produce a processor reference signal and a data signal. The present invention then uses a processor to compare the processor reference signal with the data signal by analyzing the differences in the phase and the differences in the amplitude between the two signals. The processor then extracts the fluorescence lifetime and fluorescence spectrum of the emitted signal from the phase and amplitude information using a chemometric analysis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.