Microparticle carriers of maximal uptake capacity by both M cells and non-M cells
USRE41157E1 · kind E1 · reissue
Assignee
Inventors
Key dates
| Filing date | Nov 30, 1999 |
| Grant date | Mar 2, 2010 |
| Priority date | — |
| Expiry date | Nov 30, 2019 |
Classification
- Technology area (CPC —)General
Abstract
In a solvent extraction process for preparing microspheres of a biodegradable polymer, the improvement comprising: preparing a homogenized antigen-sucrose matrix and adding a solvent to the sucrose-antigen matrix to form a solution; preparing a solution of a biodegradable polymer by adding a solvent to the polymer; adding the biodegradable polymer solution to the antigen-sucrose solution; adding an oil to the polymer-sucrose-antigen solution to form an emulsion having a controlled viscosity that corresponds to a predetermined average particle size of distributions of microspheres of biodegradable polymers; centrifuging the emulsion of controlled viscosity and removing the supernatant to obtain microspheres of a predetermined range of particle size distributions of from about 0.5 to about 7.0 micrometers.In immunostimulating composition comprising an encapsulating-microsphere of the biodegradable polymer has an average particle size distribution such that the majority of the microspheres will be taken up by the villous epithelium section of the intestines of a mammalian subject when administered as a vaccine against diseases caused by entero-pathogenic organisms. A solvent extractio…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.