Purification, composition and specificity of Heparinase I, II, and III from Flavobacterium heparinum
USRE41461E1 · kind E1 · reissue
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Key dates
| Filing date | Aug 27, 2008 |
| Grant date | Jul 27, 2010 |
| Priority date | — |
| Expiry date | Aug 27, 2028 |
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- Technology area (CPC —)General
Abstract
A single, reproducible scheme to simultaneously purify all three of the heparin lyases from F. heparinum to apparent homogeneity is disclosed herein. The kinetic properties of the heparin lyases have been determined as well as the conditions to optimize their activity and stability. Mono-clonal antibodies to the three heparinases are also described and are useful for detection, isolation and characterization of the heparinases.
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