Heterologous expression of an aspergillus kawachi acid-stable alpha amylase and applications in granular starch hydrolysis
USRE42046E1 · kind E1 · reissue
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| Filing date | Oct 23, 2008 |
| Grant date | Jan 18, 2011 |
| Priority date | — |
| Expiry date | Oct 23, 2028 |
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- Technology area (CPC —)General
Abstract
The present invention relates to a granular starch hydrolyzing enzyme composition comprising an acid stable alpha amylase (asAA) having granular starch hydrolyzing activity. The invention also relates to a one-step method for producing an alcohol which comprises contacting a granular starch substrate with an acid-stable alpha amylase (asAA) having granular starch hydrolyzing (GSH) activity and a glucoamylase (GA) in a fermentation step which also comprises ethanologenic microorganisms at a temperature of 25-40° C. to obtain a fermentation broth having 5 to 20% ethanol.
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