Covalent tethering of functional groups to proteins

USRE42931E1 · kind E1 · reissue

Assignee

• Promega Corporation · US

Inventors

• Keith V. Wood · Verona, US
• Dieter Klaubert · Arroyo Grande, US
• Georgyi V. Los · Madison, US
• Robert F. Bulleit · Verona, US
• Mark McDougall · Bethlehem, US
• Chad Zimprich · Stoughton, US

Key dates

• Filing date: Sep 17, 2008
• Grant date: Nov 15, 2011
• Expiry date: Sep 17, 2028

Classification

• Technology area (CPC —): General

Abstract

A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.