Patent · US Active

Compositions and methods for multiplex biomarker profiling

USRE47983E1 · kind E1 · reissue

31Cited by
1References
33Claims
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Key dates

Filing dateJun 28, 2018
Grant dateMay 12, 2020
Priority date
Expiry dateJun 28, 2038

Classification

  • Technology area (CPC —)General

Abstract

Provided herein are compositions and methods for identifying or quantitating one or more analytes in sample. The composition can comprise an affinity molecule reversibly conjugated to a label moiety via a double-stranded nucleic acid linker or via an adaptor molecule. The affinity molecule and the label moiety can be linked to different strands of the double-stranded nucleic acid linker. Compositions can be used in any biological assays for detection, identification and/or quantification of target molecules or analytes, including multiplex staining for molecular profiling of individual cells or cellular populations. For example, the compositions can be adapted for use in immunofluorescence, fluorescence in situ hybridization, immunohistochemistry, western blot, and the like.

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