Production cell line enhancers
USRE48651E1 · kind E1 · reissue
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10References
38Claims
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Key dates
| Filing date | Apr 17, 2019 |
| Grant date | Jul 20, 2021 |
| Priority date | — |
| Expiry date | Apr 17, 2039 |
Classification
- Technology area (CPC —)General
Abstract
The present invention relates to discovery of the ectopic expression of EDEM2 in a production cell to improve the yield of a useful multi-subunit protein. Thus, the present invention provides for production cell lines, such as the canonical mammalian biopharmaceutical production cell—the CHO cell, containing recombinant polynucleotides encoding EDEM2. Also disclosed is a production cell containing both an EDEM2-encoding polynucleotide as well an XBP1-encoding polynucleotide. Improved titers of antibodies produced by these cell lines are disclosed, as well as the improved cell densities attained by these cells in culture.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.