Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores

USRE48811E1 · kind E1 · reissue

Assignee

• The Regents of the University of California · US

Inventors

• Guillermo C. Bazan · Lompoc, US
• Brent S. Gaylord · Lompoc, US
• Shu Wang · Beijing, CN

Key dates

• Filing date: Jan 7, 2020
• Grant date: Nov 9, 2021
• Expiry date: Jan 7, 2040

Classification

• Technology area (CPC —): General

Abstract

Methods compositions and articles of manufacture for assaying a sample for a target polynucleotide are provided. A sample suspected of containing the target polynucleotide is contacted with a polycationic multichromophore and a sensor polynucleotide complementary to the target polynucleotide. The sensor polynucleotide comprises a signaling chromophore to receive energy from the excited multichromophore and increase emission in the presence of the target polynucleotide. The methods can be used in multiplex form. Kits comprising reagents for performing such methods are also provided.