Method for preparing for and carrying out the acquisition of image stacks of a sample from various orientation angles

US10001634B2 · kind B2 · utility

Assignee

• Carl Zeiss Microscopy GmbH · DE

Inventors

• Helmut Lippert · Jena, DE
• Olaf Selchow · Jena, DE
• Pavel Tomancak · Dresden, DE

Key dates

• Filing date: Jun 24, 2013
• Grant date: Jun 19, 2018
• Expiry date: Feb 23, 2034

Classification

• Technology area (CPC G): Physics
• CPC primary: G02B21/367
• WIPO field: Optics
• WIPO sector: Instruments

Abstract

A method for preparing for and carrying out the microscopic recording of image stacks of a sample from various orientation angles, wherein the sample is retained in a sample retainer defining a sample coordinate system. The sample retainer can be translated and rotated in a space spanned by a detection coordinate system. In a first embodiment, for at least two different orientation angles, a projection image is recorded in the detection coordinate system and a first sample volume and a second sample volume are determined in the sample coordinate system. Then the intersection volume of the two sample volumes is determined in the sample coordinate system. Parameters necessary for image stack recording are determined for all other orientation angles based on the intersection volume.