Method for metabolomic sample preparation based on ionic liquid dispersive liquid-liquid microextraction
US10012574B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Mar 11, 2014 |
| Grant date | Jul 3, 2018 |
| Priority date | — |
| Expiry date | Jan 7, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2570/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided herein is a method comprising one or more of the following steps: (a) lysing cells of a biological sample and contacting the biological sample with an amount of ionic liquid sufficient to denature intracellular metabolic enzymes in the biological sample to produce a contacted cellular sample; (b) mixing the contacted cellular sample with an organic solvent to produce an ionic liquid-organic solvent composition; (c) mixing the contacted cellular sample with the organic solvent to produce a dispersed microdroplet ionic liquid-organic solvent composition; (d) contacting the ionic liquid-organic solvent composition with an ion exchange composition to produce a second ionic liquid-organic solvent composition; (d) separating the ionic liquid from the organic solvent; and (e) extracting metabolites from the ionic liquid. Kits and systems for practicing the subject methods are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.