Methods and systems for biological instrument calibration
US10012590B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 5, 2016 |
| Grant date | Jul 3, 2018 |
| Priority date | — |
| Expiry date | Jun 14, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/922
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
In one exemplary embodiment, a method for calibrating an instrument is provided. The instrument includes an optical system capable of imaging florescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration to determine reaction site positions in an image. The method further includes performing a pure dye calibration to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration to determine a filter normalization factor. The method includes performing an RNase P validation to validate the instrument is capable of distinguishing between two different quantities of sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.