Patent · US Active

Method and arrangement for light sheet microscopy

US10031325B2 · kind B2 · utility

2Cited by

0References

12Claims

0Family size

Assignee

Carl Zeiss Microscopy GmbH · DE

Inventors

Tiemo Anhut · Jena, DE
Thomas Kalkbrenner · Jena, DE
Daniel Schwedt · Weimar, DE
Jörg Siebenmorgen · Jena, DE
Helmut Lippert · Jena, DE

Key dates

Filing date	Feb 19, 2015
Grant date	Jul 24, 2018
Priority date	—
Expiry date	Feb 19, 2035

Classification

Technology area (CPC H)Electricity
CPC primaryH04N25/71
WIPO fieldAudio-visual technology
WIPO sectorElectrical engineering

Abstract

An arrangement for light sheet microscopy that includes a means for scanning a sample volume with a light sheet, which includes an angle δ≠90° with the optical axis of an objective. The light sheet passes through the entire sample volume in the propagation direction, and the depth of field Sobj of the objective is less than the optical-axis depth T of this sample volume. An optical device, disposed downstream of the objective, increases the depth of field Sobj to a depth of field Seff≥the depth T of this sample volume. The arrangement also includes a means for positioning the sample volume within the region of the depth of field Seff. A spatially resolving optoelectronic area sensor is disposed downstream of the optical device, and hardware and software are provided to generate sample-volume images from the electronic image signals output by the area sensor.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.