Methods of producing two chain proteins in bacteria
US10066002B2 · kind B2 · utility
3Cited by
43References
27Claims
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Key dates
| Filing date | Nov 5, 2015 |
| Grant date | Sep 4, 2018 |
| Priority date | — |
| Expiry date | Nov 5, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein are methods of producing a recombinant polypeptide containing two chains, such as an immune mobilizing monoclonal T-cell receptor against cancer (ImmTAC) protein including an alpha chain and a beta chain. In particular, methods are provided for producing heterologous secretory proteins in bacteria through utilization of optimized expression vectors and culture processes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.