Yeast cells having NADP(H)-dependent reductive TCA pathway from pyruvate to succinate

US10066246B2 · kind B2 · utility

Assignee

• CARGILL, INCORPORATED · US

Inventors

• Brian J. Rush · Minneapolis, US
• Kevin T. Watts · Minneapolis, US
• Vernon L. McIntosh, Jr. · Minneapolis, US
• Arlene M. Fosmer · Eden Prairie, US
• Gregory M. Poynter · Windom, US
• Thomas W. McMullin · Manitowoc, US

Key dates

• Filing date: Jul 25, 2013
• Grant date: Sep 4, 2018
• Expiry date: Dec 3, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y604/01001
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Recombinant yeast cells contain a reductive TCA pathway from phosphoenolpyruvate or pyruvate to succinate. At least one metabolic step in the pathway includes a reaction of NADPH to produce NADP+. The yeast cell contains at least one exogenous NADPH-dependent gene in the pathway from phosphoenolpyruvate or pyruvate to succinate, preferably an NADPH-dependent malate dehydrogensase or fumarate reducase gene (or both).