Microfluidic microbe detection and isolation

US10077462B2 · kind B2 · utility

Assignees

• The Broad Institute, Inc. · US
• Massachusetts Institute of Technology · US
• The General Hospital Corporation · US

Inventors

• Han Wei Hou · Singapore, SG
• Jongyoon Han · Ithaca, US
• Roby Bhattacharyya · Cambridge, US
• Deb HUNG · Lexington, US

Key dates

• Filing date: Mar 14, 2014
• Grant date: Sep 18, 2018
• Expiry date: Sep 6, 2034

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2015/0288
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method and microfluidic device useful for isolating microbes from a blood sample which includes introducing the blood sample into the sample inlet of a spiral microfluidic device; and introducing a second fluid into the sheath inlet of the microfluidic device, wherein the spiral channel terminates in a microbe outlet and a waste outlet, and wherein the spiral channel includes a length, height, and a width that define an aspect ratio adapted to isolate any microbes present in the sample along a first portion of the spiral channel terminating at the microbe outlet, and to isolate red blood cells and leukocytes along a second portion of the spiral channel terminating at the waste outlet; and collecting the microbes from the microbe outlet, thereby isolating the microbes.