Patent · US Active

Methods for multiplexing amplification reactions

US10106845B2 · kind B2 · utility

1Cited by
53References
20Claims
0Family size

Assignee

Inventors

Key dates

Filing dateOct 14, 2016
Grant dateOct 23, 2018
Priority date—
Expiry dateOct 14, 2036

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q2600/16
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.