Polypeptides targeting glycosylated MUC2 proteins, methods of synthesis, their nucleic acids and uses thereof

US10138280B2 · kind B2 · utility

Assignee

• INSTITUT PASTEUR · FR

Inventors

• Benoit Marteyn · Paris, FR
• Yves-Marie Coic · Meudon, FR
• Francoise Baleux · Paris, FR
• Philippe Sansonetti · Paris, FR

Key dates

• Filing date: Jun 9, 2016
• Grant date: Nov 27, 2018
• Expiry date: Jun 21, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/61
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention relates to polypeptides, defined through a consensus sequence, having a length from 10 to 80 amino-acid residues, and whose polypeptidic sequence comprises or consists of the consensus sequence P1(Xa)P3(Xb)P5(Xc)P6(Xd)P7 (SEQ ID NO: 1), presenting specific patterns. The polypeptides of the invention target glycosylated Muc2 proteins. The invention also relates to methods of synthesis of such polypeptides, to their nucleic acids and uses thereof. The polypeptidic sequence of the polypeptides of the invention can be part of the N-terminal sequence of a mucus-binding (MUB) domain, especially a mucus-binding (MUB) domain of several species. The invention also relates to chimeric molecule(s) comprising such polypeptides, which are labelled, and vectors, especially plasmids and population of cells or composition comprising polypeptides of the invention. Synthesis methods encompass biotechnological or chemical production. Polypeptides of the invention can be used in staining experiments, as a probe or marker for staining Muc2 protein(s) contained in mucus layer(s), to detect in vitro mucus production or mucus composition in human colon or monitoring any one of the following …