Patent · US Active

Cell concentration adjustment device, and automatic subculture system using same

US10138456B2 · kind B2 · utility

1Cited by
0References
8Claims
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Assignee

Inventors

Key dates

Filing dateJul 8, 2015
Grant dateNov 27, 2018
Priority date
Expiry dateJul 8, 2035

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/06
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A device which automatically performs a step in which expanded and cultured cells are diluted to a desired cell concentration and re-inoculated using a cell-concentration adjustment device having an inlet for taking in a cell suspension; an outlet for discharging a diluted cell suspension; and a flow path which is provided between the inlet and the outlet and is capable of holding a cell suspension, the flow path being provided with: a liquid delivery pump for causing a cell suspension inside to flow; a cell-concentration measurement instrument for collecting data related to a cell concentration per unit amount of the cell suspension; and a dilution-liquid container for holding a dilution liquid which is supplied to the flow path to dilute the cell suspension. The device further includes a control unit for controlling at least the liquid delivery pump on the basis of the data obtained by the cell-concentration measurement instrument, wherein the control unit determines, on the basis of the data obtained by the cell-concentration measurement instrument, an amount of the dilution liquid required to bring the cell concentration to the desired concentration, and drives the liquid deliv…

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.