Method for generating a contrast image of an object structure and apparatuses relating thereto

US10175468B2 · kind B2 · utility

Assignee

• Carl Zeiss Microscopy GmbH · DE

Inventors

• Lars Stoppe · Jena, DE
• Christoph Husemann · Jena, DE

Key dates

• Filing date: Apr 29, 2016
• Grant date: Jan 8, 2019
• Expiry date: Jan 1, 2037

Classification

• Technology area (CPC G): Physics
• CPC primary: G06T2207/10152
• WIPO field: Audio-visual technology
• WIPO sector: Electrical engineering

Abstract

The invention relates to a contrast formation method, for generating a contrast image, including the steps: Illuminating an object by an illumination sequence based on one or more illumination sources; creating an illumination image of the object for each illumination in the illumination sequence; overlaying two respective illumination images which neighbor each other with respect to a first axis to form a first total axis image of the first axis; overlaying two respective illumination images which neighbor each other with respect to a second axis to form a second total axis image of the second axis; creating a first color gradient image based on the first total axis image; creating a second color gradient image based on the second total axis image; transforming the first color gradient image and the second color gradient image into a color space; and generating a contrast image, based on the transformed first color gradient image and the second color gradient image into the color space.