Patent · US Active

Methods for enzyme mediated polypeptide conjugation

US10190182B2 · kind B2 · utility

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15Claims

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Assignee

Hoffmann-La Roche Inc. · US

Inventors

Mara Boenitz-Dulat · Tutzing, DE
Peter Kratzsch · Penzberg, DE
Martin Schatte · Waldbronn, DE

Key dates

Filing date	Jun 16, 2017
Grant date	Jan 29, 2019
Priority date	—
Expiry date	Jul 28, 2037

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K2317/52
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Herein is reported a method for producing an enzymatic conjugation product of two polypeptides comprising incubating of a first polypeptide comprising the amino acid sequence LPXTG (SEQ ID NO: 20, wherein X can be any amino acid residue), a second polypeptide has an oligo-alanine Am (m=2 (SEQ ID NO: 26), or 3 (SEQ ID NO: 27), or 4 (SEQ ID NO: 28), or 5 (SEQ ID NO: 29)) amino acid sequence at its N-terminus, a third polypeptide with sortase activity which is derived from Staphylococcus aureus Sortase A, and recovering the conjugate from the reaction mixture and thereby producing the enzymatic conjugation product of two polypeptides.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.