Method for biotechnological production of methylized cinnamic acids and cinnamic acid esters, methylized phenethylamines and the coupling products thereof, particularly of cinnamic acid amides

US10227620B2 · kind B2 · utility

Assignee

• SYMRISE AG · DE

Inventors

• Gerhard Krammer · Holzminden, DE
• Jakob Ley · Holzminden, DE
• Katrin Geißler · Einbeck, DE
• Torsten Geiler · Augsburg, DE
• Frauke Gomoll · Sankt Ingbert, DE
• Peter Welters · Nettetal, DE
• Guido Jach · Neunburg vorm Wald, DE
• Ludger A. Wessjohann · Bodenwerder, DE

Key dates

• Filing date: Feb 1, 2016
• Grant date: Mar 12, 2019
• Expiry date: Sep 28, 2036

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A23V2002/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to providing both fermentative and biotechnological methods for producing 3,4-methylized cinnamic acids, 3,4-methylized cinnamic acid esters, 3,4-dimethoxyphenethylamine, and 4-methylized cinnamic acid amides using a 4′-O-methyltransferase, optionally in combination with further enzymes, wherein the enzymes are selected by means of metabolic engineering and operation have been adapted by targeted optimization, and compositions obtained by means of the method.The invention further relates to vector systems, recombinant microorganisms or fungi, and specific nucleic acid segments and polypeptides.