Methods and compositions for isothermal amplification and detection of mycoplasma pneumoniae

US10233504B2 · kind B2 · utility

Assignees

• The United States of America, as represented by the Secretary, Department of Health and Human Services · US
• P3S Corporation · US

Inventors

• Jonas M. Winchell · Lilburn, US
• Brianna Petrone · Durham, US
• Maureen Diaz · Atlanta, US
• Bernard J. Wolff · Roswell, US

Key dates

• Filing date: Feb 12, 2016
• Grant date: Mar 19, 2019
• Expiry date: Oct 7, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6844
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed herein are methods and compositions (e.g., oligonucleotide primers) for isothermal amplification and detection of M. pneumoniae nucleic acids in a sample. In some embodiments, the methods include contacting a sample with a set of LAMP primers specific for a M. pneumoniae CARDS toxin-encoding nucleic acid under conditions sufficient to produce an M. pneumoniae nucleic acid amplification product and detecting the resulting M. pneumoniae amplification product. Kits including sets of LAMP primers for detection of M. pneumoniae CARDS toxin nucleic acids are also provided herein.