Patent · US Active

Method for purifying PEGylated erythropoietin

US10273277B2 · kind B2 · utility

1Cited by

5References

9Claims

0Family size

Assignee

Hoffmann-La Roche Inc. · US

Inventors

Roberto Falkenstein · Munich, DE
Wolfgang KOEHNLEIN · Penzberg, DE
Wolfgang Kuhne · Penzberg, DE
Hartmut Schurig · Munich, DE

Key dates

Filing date	Feb 19, 2016
Grant date	Apr 30, 2019
Priority date	—
Expiry date	Feb 19, 2036

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY02P20/582
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

Herein is reported a method for the purification of a protein comprising erythropoietin and a single poly (ethylene glycol) residue from reaction by-products or not reacted starting material by a cation exchange chromatography method. It has been found that by employing a cation exchange SP Sephacryl™ S 500 HR chromatography material conditioned to a conductivity of 21 mS/cm and a linear gradient elution a fusion protein of erythropoietin and a single poly (ethylene glycol) residue can be obtained in a single step with high purity and yield.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.