Target enrichment and labeling for multi-kilobase DNA
US10308979B2 · kind B2 · utility
1Cited by
2References
17Claims
0Family size
Assignee
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Key dates
| Filing date | Mar 14, 2013 |
| Grant date | Jun 4, 2019 |
| Priority date | — |
| Expiry date | Sep 4, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6806
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This disclosure provides a method comprising: a) clamping the top and bottom strands of a double stranded DNA molecule to produce a duplex in which the top and bottom strands are linked; b) denaturing the duplex to produce a denatured product; and c) renaturing the denatured product in the presence of a labeled oligonucleotide that is complementary to a sequence of nucleotides in the double stranded DNA molecule, thereby producing a D-loop-containing product. Kits for performing the method and products made by the method are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.