Structurally designed shRNAs
US10329562B2 · kind B2 · utility
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19Claims
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Key dates
| Filing date | Mar 6, 2017 |
| Grant date | Jun 25, 2019 |
| Priority date | — |
| Expiry date | Mar 6, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2320/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided is an improved design of shRNA based on structural mimics of miR-451 precursors. These miR-451 shRNA mimics are channeled through a novel small RNA biogenesis pathway, require AGO2 catalysis and are processed by Drosha but are independent of DICER processing. This miRNA pathway feeds active elements only into Agog because of its unique catalytic activity. These data demonstrate that this newly identified small RNA biogenesis pathway can be exploited in vivo to produce active molecules.
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