Stabilized influenza hemagglutinin stem region trimers and uses thereof

US10363301B2 · kind B2 · utility

Assignee

• The United States of America, as represented by the Secretary, Department of Health and Human Services · US

Inventors

• John Mascola · Rockville, US
• Jeffrey Boyington · Clarksburg, US
• Hadi M. Yassine · Doha, QA
• Peter Kwong · Washington, US
• Barney Graham · Rockville, US
• Masaru Kanekiyo · Bethesda, US

Key dates

• Filing date: May 27, 2015
• Grant date: Jul 30, 2019
• Expiry date: May 27, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2760/16134
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Vaccines that elicit broadly protective anti-influenza antibodies. Some vaccines comprise nanoparticles that display HA trimers from influenza virus on their surface. The nanoparticles are fusion proteins comprising a monomeric subunit (e.g., ferritin) joined to the stem region of an influenza HA protein. The fusion proteins self-assemble to form the HA-displaying nanoparticles. The vaccines comprise only the stem region of an influenza HA protein joined to a trimerization domain. Also provided are fusion proteins, and nucleic acid molecules encoding such proteins, and assays using nanoparticles of the invention to detect anti-influenza antibodies.