Single-cell raman spectroscopy for the non-destructive, non-invasive analysis of cells and cellular components

US10365220B2 · kind B2 · utility

Assignee

• Lawrence Livermore National Security, LLC · US

Inventors

• James W. Chan · Elk Grove, US
• Thomas R. Huser · Werther, DE
• Stephen M. Lane · Oakland, US
• John C. Rutledge · Davis, US
• Douglas S. Taylor · Davis, US
• Theodore Zwerdling · Davis, US
• Ronald A. Li · Kildeer, US

Key dates

• Filing date: May 31, 2006
• Grant date: Jul 30, 2019
• Expiry date: Nov 21, 2030

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2021/656
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Raman spectra of cells, such as normal human T- and B-cells from peripheral blood or human tonsil and the corresponding transformed cells are obtained by optically trapping the cells and obtaining their Raman spectra. The trapped cells can be subjected to one, two, or more different excitation wavelengths, and each wavelength of the corresponding Raman spectra can be stored in a separate channel. In preferred embodiments, two spectra are subtracted from each other to give a difference spectrum and each channel is analyzed independently to characterize the trapped cell. Alternatively, the Raman spectrum can be subjected to Principal Component Analysis (PCA) in order to characterize the trapped cell. The trapped cell thus classified can be sorted, or further manipulated.